Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method |
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| Authors: | Rubiano Claudia C Wasserman Moises |
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| Affiliation: | Laboratorio Bioquímica, Instituto Nacional de Salud, Bogotá, Colombia. |
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| Abstract: | A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells. |
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