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Stable transformation of lettuce cultivar South Bay from cotyledon explants
Authors:A C Torres  D J Cantliffe  B Laughner  M Bienick  R Nagata  M Ashraf  R J Ferl
Institution:(1) Vegetable Crops Department, University of Florida, IFAS, P.O. Box 110690, 32611-0690 Gainesville, FL, USA;(2) Present address: EMBRAPA/CNPH, P.O. Box 07-0218, 70359 Brasilia, DF, Brazil;(3) Present address: Plant Molecular Biology Center, Northern Illinois University, 60115 DeKalb, IL, USA
Abstract:Transgenic plants of lettuce cultivar (cv.) lsquoSouth Bayrsquo were produced by using Agrobacterium tumefaciens vectors containing the beta-glucuronidase (GUS) reporter gene and the NPT II gene for kanamycin resistance as a selectable marker. High frequency of transformation, based on kanamycin resistance and assays for GUS expression, was obtained with 24 to 72-h-old cotyledon explants cocultivated for 48 h with Agrobacterium tumefaciens. After the cocultivation period, the explants were placed in selection medium containing 50 or 100 mg l–1 of kanamycin, 100 mg l–1 cefotaxime and 500 mg l–1 carbenicillin for 10 days. Surviving explants were transferred every 14 days on shoot elongation medium. Progenies of R0 plants demonstrated linked monogenic segregation for kanamycin resistance and GUS activity.Florida Agricultural Experiment Station Journal Series R-02231. This research was partially supported by CNPq/RHAE (Brazil).
Keywords:Lactuca sativa  regeneration  tissue culture  transgenic plant
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