Molecular character of the recombinant antitumor lectin from the edible mushroom Agrocybe aegerita |
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Authors: | Yang Na Tong Xin Xiang Ye Zhang Ying Liang Yi Sun Hui Wang Da-Cheng |
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Affiliation: | Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing. |
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Abstract: | The lectin from Agrocybe aegerita (AAL) has been found to possess potent tumor-suppressing function and tumor cell apoptosis-inducing activity. In this paper, we report the full sequence, the active expression of the gene encoding AAL at a high level and bioassay of the binding property with lactose, apoptosis-inducing activity and DNase activity of recombinant AAL (rAAL). The results reveal that AAL is a member of the galectin family and the dimeric form is the active unit for the functional performance. The rAAL showed comparable tumor cell apoptosis-inducing activity with the wild AAL but no DNase activity at all. The molecular characters revealed by this study are significant for the in-depth investigation of the functional mechanism of this interesting protein. |
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