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Antimicrobial Activities and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of <Emphasis Type="Italic">Bacillus</Emphasis> Isolates from the Marine Sponge <Emphasis Type="Italic">Aplysina aerophoba</Emphasis> |
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| Authors: | Christian T?Pabel Joachim?Vater Christopher?Wilde Peter?Franke Jürgen?Hofemeister Barbara?Adler Gerhard?Bringmann J?rg?Hacker |
| Institution: | (1) Institut für Molekulare Infektionsbiologie, Universität Würzburg, Röntgenring 11, D-97070 Würzburg, Germany;(2) Institut für Chemie, Arbeitsgruppe Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrabe 29, D-10587 Berlin, Germany;(3) Institut für Biochemie, Freie Universität Berlin, Thielallee 63, D-14195 Berlin, Germany;(4) Institut für Pflanzengenetik und Kulturpflanzenforschung, Abteilung Molekulare Genetik, Corrensstrabe 3, D-06466 Gatersleben, Germany;(5) Institut für Organische Chemie, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany |
| Abstract: | The aim of this study was to isolate bacteria that are resistant to the strong antimicrobial metabolites characteristic of Aplysina aerophoba. For this purpose, bacterial isolation was performed on agar plates to which sponge tissue extract had been added. Following screening for antifungal and antimicrobial activities, 5 strains were chosen for more detailed analyses. 16S ribosomal DNA sequencing revealed that all isolates belonged to the genus Bacillus, specifically B. subtilis and B. pumilus. Using a combination of matrix-assisted laser desorption/ ionization mass spectrometry typing of whole cells and antimicrobial bioassays against selected reference strains, the bioactive metabolites were identified as lipopeptides. |
| Keywords: | MALDI MS Bacillus sponge antimicrobial lipopeptide iturin |
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