Ligninolytic system of Phanerochaete chrysosporium: Inhibition by o-Phthalate |
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Authors: | Patrick Fenn T. Kent Kirk |
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Affiliation: | (1) Forest Service, Forest Products Laboratory, U.S. Department of Agriculture, P.O. Box 5130, 53705 Madison, WI, USA |
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Abstract: | The degradation rate of [synthetic-14C]-lignin to 14CO2 by Phanerochaete chrysosporium in cultures buffered with 0.01 M 2,2-dimethylsuccinate (DMS) was twice that in 0.01 M o-phthalate-buffered cultures. This difference could be totally accounted for by o-phthalate inhibition of the activity of the ligninolytic system. 14CO2 production from ring-, sidechain-, and methoxyl-labeled lignins was inhibited, the degree of inhibition being dependent on o-phthalate concentration. Oxidations of 14C-glucose, 14C-acetovanillone, and 14C-apocynol were not inhibited; thus o-phthalate is not a general inhibitor, and might inhibit activities involved in attack of the lignin polymer. DMS is a suitable buffer for the ligninolytic system. Degradation rates of ring-labeled lignin to 14CO2 of 10–15% in 24 h were obtained consistently over the pH range 3.6–4.5, with an optimum near pH 4.0.Non-Standard Abbreviations DMS dimethylsuccinate |
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Keywords: | 2,2-Dimethylsuccinate o-Phthalate Buffer Inhibition Lignin biodegradation Phanerochaete chrysosporium |
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