<Emphasis Type="Italic">Xenopus</Emphasis> importin beta validates human importin beta as a cell cycle negative regulator |
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Authors: | Valerie A Delmar Rene C Chan Douglass J Forbes |
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Institution: | (1) Section of Cell and Developmental Biology, Division of Biological Sciences 0347, University of California - San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0347, USA;(2) Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA |
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Abstract: | Background Human importin beta has been used in all Xenopus laevis in vitro nuclear assembly and spindle assembly studies. This disconnect between species raised the question for us as to whether importin
beta was an authentic negative regulator of cell cycle events, or a dominant negative regulator due to a difference between
the human and Xenopus importin beta sequences. No Xenopus importin beta gene was yet identified at the time of those studies. Thus, we first cloned, identified, and tested the Xenopus importin beta gene to address this important mechanistic difference. If human importin beta is an authentic negative regulator
then we would expect human and Xenopus importin beta to have identical negative regulatory effects on nuclear membrane fusion and pore assembly. If human importin
beta acts instead as a dominant negative mutant inhibitor, we should then see no inhibitory effect when we added the Xenopus homologue. |
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