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Identification of nine sequence types of the 16S rRNA genes of <Emphasis Type="Italic">Campylobacter jejuni</Emphasis> subsp. <Emphasis Type="Italic">jejuni</Emphasis> isolated from broilers
Authors:Ingrid Hansson  Marianne Persson  Linda Svensson  Eva Olsson Engvall  Karl-Erik Johansson
Institution:(1) Department of Bacteriology, National Veterinary Institute, SE-751 89 Uppsala, Sweden;(2) Swedish Zoonosis Center, Department of Disease Control, National Veterinary Institute, SE-751 89 Uppsala, Sweden;(3) Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
Abstract:

Background  

Campylobacter is the most commonly reported bacterial cause of enteritis in humans in the EU Member States and other industrialized countries. One significant source of infection is broilers and consumption of undercooked broiler meat. Campylobacter jejuni is the Campylobacter sp. predominantly found in infected humans and colonized broilers. Sequence analysis of the 16S rRNA gene is very useful for identification of bacteria to genus and species level. The objectives in this study were to determine the degree of intraspecific variation in the 16S rRNA genes of C. jejuni and C. coli and to determine whether the 16S rRNA sequence types correlated with genotypes generated by PFGE analysis of Sma I restricted genomic DNA of the strains.
Keywords:
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