|
|||||
|
|
Fluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae |
|
| Authors: | Juan Carlos Aon Sonia Cortassa |
| Institution: | Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT), Departamento de Bioquímica de la Nutrición, Instituto de Química Biológica 'Dr. BernabéBloj', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina;Instituto Tecnológico de Chascomús (INTECH), Casilla Correo 164, 7130 Chascomús, Buenos Aires, Argentina |
| Abstract: | This work reports the intracellular pH (pHi) dynamics of Saccharomyces cerevisiae cells in sporulation medium. Cells loaded with the pH-sensitive dye carboxy-seminaphthorhodafluor-1 (C.SNARF-1) exhibited an alkalization of the pHi following the extracellular pH during sporulation in the absence of buffer and almost no change in pHi or ΔpH when sporulation was carried out in buffered medium. The results indicate that the pH gradient does not appear to be directly involved in the regulation of acetate uptake during sporulation. However, the alkalization of pHi by eliciting a decrease in metabolic fluxes could account for a lower demand for acetate. |
| Keywords: | Saccharomyces cerevisiae Fluorescent dye Intracellular pH Sporulation |
| 本文献已被 ScienceDirect 等数据库收录！ | |