In situ monitoring of DNA: the plant nuclear envelope allows passage of short DNA fragments |
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| Authors: | Andreas Gisel Barbara Rothen Victor A. Iglesias Ingo Potrykus Christ of Sautter |
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| Affiliation: | ;Institute of Plant Sciences, ETH-Zürich, Universitätsstr. 2, CH-8092 Zürich, Switzerland, and ;Institute of Cell Biology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland |
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| Abstract: | We monitored the cellular localization of fluorescently labeled foreign DNA in living plant cells. After physical delivery of labeled DNA fragments to the cytoplasm, short fragments up to 1.5 kb in length were found equally distributed between the cytoplasm and nucleus after 60 min. In contrast, 2.5 kb DNA fragments did not appear inside the nucleus. Thus, foreign DNA can enter plant nuclei through the intact nuclear envelope, but the efficiency of this process declines with increasing size of fragment. |
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