Neutral microprotein, a novel human plasma and urinary protein associated with a yellow-brown chromophore. Isolation from protein HC preparations and partial characterization |
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Authors: | C López-Otin A O Grubb E Méndez |
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Affiliation: | 1. Servicio de Endocrinologia, Centro “Ramon y Cajal”, Carretera de Colmenar, Km. 9.1, Madrid-34, Spain;2. Department of Clinical Chemistry, University of Lund, Malmö General Hospital, S-214 01 Malmö, Sweden |
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Abstract: | An apparently novel human plasma and urinary protein of low molecular weight was isolated from several highly purified preparations of protein HC by gel chromatography and high voltage electrophoresis with a yield of about 8 mg/g. The protein has a molecular weight of about 20,000, neutral electrophoretic mobility at pH 6.5 and a high content of half-cystine. It is associated with a yellow-brown chromophore like protein HC and could be demonstrated in all investigated preparations of isolated human, rabbit and guinea-pig protein HC and alpha 1-microglobulin. |
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