Anthocyanins from soybean seed coat inhibit the expression of TNF-alpha-induced genes associated with ischemia/reperfusion in endothelial cell by NF-kappaB-dependent pathway and reduce rat myocardial damages incurred by ischemia and reperfusion in vivo |
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Authors: | Kim Hye Jung Tsoy Irina Park Jung Min Chung Jong Il Shin Sung Chul Chang Ki Churl |
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Institution: | Department of Pharmacology, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Republic of Korea. |
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Abstract: | We examined the inhibition of the expression of some inflammatory genes associated with ischemia-reperfusion (I/R) injury by anthocyanins isolated from black soybean seed coat in tumor necrosis factor-alpha (TNF-alpha)-treated bovine aortic endothelial cells. In addition, its potential use on I/R-injury was investigated using rats subjected to 30-min occlusion of left descending coronary artery followed by 24-h reperfusion. Western blot analysis and luciferase activity assay showed that anthocyanins inhibited TNF-alpha-induced vascular cell adhesion molecule-1, intracellular adhesion molecule-1, and cyclooxygenase-2 levels, which is through NF-kappaB-dependent pathway. Further, anthocyanins protected myocardiac injury from I/R in rats. It is suggested that anthocyanins from black soybean seed coat can be used as a useful drug to modulate cardiovascular disorder. |
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Keywords: | COX-2 cylooxygenase-2 ICAM-1 intracellular adhesion molecule-1 I/R ischemic reperfusion VCAM-1 vascular adhesion molecule-1 TBS-T Tris-buffered saline/Tween 20 |
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