Volatile acid production from threonine,valine, leucine and isoleucine by clostridia |
| |
Authors: | Sidney R Elsden Martin G Hilton |
| |
Institution: | (1) Agricultural Research Council Food Research Institute, Colney Lane, NR4 7UA Norwich, UK;(2) Present address: School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, UK |
| |
Abstract: | The amounts of the volatile acids produced from thereonine, valine, leucine and isoleucine by growing cultures of clostridia have been measured. The species used were Clostridium sporogenes; C. caloritolerans; C. botulinum proteolytic type A; C. botulinum proteolytic type B; C. botulinum proteolytic type F; C. botulinum proteolytic type G; C. putrificum; C. difficile; C. ghoni; C. bifermentans; C. sordellii; C. mangenoti; C. cadaveris; C. lituseburense; C. propionicum; C. sticklandii; C. scatologenes; C. subterminale; C. putrefaciens; C. histolyticum; C. tetanomorphum; C. limosum; C. lentoputrescens; C. tetani; C. melanomenatum; C. cochlearium; C. sporospheroides. Most of the species tested gave increased yields of propionic acid when grown in the threonine medium; in addition, some species resembled C. propionicum and produced n-butyric acid when grown in this medium. C. histolyticum produced only acetic acid in the basal medium; all seven strains of this species produced more acetic acid when grown in the threonine medium than in the basal medium. Species which oxidize valine to iso-butyric acid also oxidize leucine to 3-methyl butyric acid and isoleucine to 2-methylbutyric acid. The iso-caproic fraction produced by some species is shown to be derived from leucine. The identitity of the branched-chain acids produced by C. sporogenes has been confirmed by gas liquid chromatography/mass spectrometry.Abbreviations GLC
gas liquid chromatography
- RCM
reinforced clostridial medium
- VFA
volatile fatty acid |
| |
Keywords: | Clostridia Threonine Valine Leucine Isoleucine Propionic acid iso-butyric acid n-butyric acid 2-methyl butyric acid 3-methyl butyric acid 4-methyl valeric acid |
本文献已被 PubMed SpringerLink 等数据库收录! |
|