Immunohistochemistry of carbonic anhydrase isozymes VI and II during development of the rat salivary glands |
| |
Authors: | Y Ogawa Ross T Fernley Reiko Ito Naokuni Ijuhin |
| |
Institution: | (1) Department of Oral Pathology, Osaka University, Faculty of Dentistry, 1–8 Yamadaoka, Suita, Osaka, 565 Japan Tel.: +81-6-879-2892; fax +81-6-879-2895 e-mail ogawa@dent.osaka-u.ac.jp, JP;(2) Biomolecular Research Institute, Victoria, Australia, AU |
| |
Abstract: | Secreted carbonic anhydrase (isozyme VI; CA VI) was localized by immunohistochemistry in the developing postnatal rat submandibular
and parotid glands using a specific monoclonal antibody to the rat enzyme. CA VI immunostaining was not detectable in the
glands before birth. In the submandibular gland, granular immunostaining for CA VI was detectable in several terminal tubule
cells of 1-day-old rats. At 1 week, the CA VI-positive cells were located at the periphery of the terminal tubules and appeared
to be budding off the tubules. These cellular buds gradually increased, and, by 4 weeks, formed acini. CA VI was also detected
in the duct lumen from day 1. The immunostaining in the parotid gland was detected sporadically in the acinar cells at 2 or
3 weeks. By 4 weeks, when the gland was almost indistinguishable from the adult one, the number of positive acinar cells had
increased. Their number, however, was far smaller than in the adult gland, and the enzyme could not be detected in the duct
lumen. CA II was also localized using specific antibodies to the rat isozyme. CA II was detectable in the inter- and intralobular
striated ducts at 2 weeks after birth in the submandibular gland and at 3 weeks in the parotid gland. These results suggset
that CA VI is secreted into saliva from soon after birth and that CA II appears in parallel with the functional maturation
of the ducts. In addition, CA II was transiently expressed by the cellular buds of the submandibular gland at 2 and 3 weeks.
Accepted: 7 January 1998 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|