| 胆红素氧化酶产酶菌株的分离及最佳产酶条件的研究 |
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| 引用本文： | 郭健 陶沙 莫培生 粱锡娴 黎高翔. 胆红素氧化酶产酶菌株的分离及最佳产酶条件的研究[J]. 微生物学报, 1991, 31(2): 156-159 |
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| 作者姓名： | 郭健 陶沙 莫培生 粱锡娴 黎高翔 |
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| 作者单位： | 中国科学院微生物研究所 北京；卫生部临床检验中心 北京 |
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| 摘 要： |
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| 关 键 词： | 胆红素氧化酶 产酶菌 分离 |
Isolation of bilirubin oxidase from Myrothecium verrucaria and the optimum conditions of enzyme production |
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| J Guo,S Tao,P S Mo,X Liang,G Li. Isolation of bilirubin oxidase from Myrothecium verrucaria and the optimum conditions of enzyme production[J]. Acta microbiologica Sinica, 1991, 31(2): 156-159 |
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| Authors: | J Guo S Tao P S Mo X Liang G Li |
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| Affiliation: | National Center for Clinical Laboratory, Beijing. |
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| Abstract: | A bilirubin oxidase (EC 1.3.3.5) producing strain, Mv 2.1089, was isolated from several strains of Myrothecium verrucaria by dilution method. The optimum conditions of enzyme production were investigated and the results were as follows: the suitable medium was cultured at 25 degrees C on a rotating shaker glucose and peptone, at pH 6.0. The strain was cultured at 25 degrees C on a rotating shaker (150 r/min) for 96 h. Bilirubin oxidase with 0.5-1.5 u/ml was obtained in the culture medium. |
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