| 球形芽孢杆菌TS—1原生质体电诱导质粒转化研究 |
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| 引用本文： | 王岳五,江波.球形芽孢杆菌TS—1原生质体电诱导质粒转化研究[J].微生物学报,1991,31(2):94-99. |
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| 作者姓名： | 王岳五 江波 |
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| 摘 要： |
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| 关 键 词： | 球形芽孢杆菌 原生质体 电击转化 |
The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation] |
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| Y Wang,B Jiang,R Jiao.The study of Bacillus sphaericus Ts-1 protoplast-plasmid transformation of electroporation][J].Acta Microbiologica Sinica,1991,31(2):94-99. |
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| Authors: | Y Wang B Jiang R Jiao |
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| Institution: | Department of Biology, Nankai University, Tianjin. |
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| Abstract: | This report gave the best conditions of Bacillus sphaericus Ts-1 protoplast-plasmid pHV33 electroporation. The highest transformation frequency and transformation efficiency induced by three pulse of 21 KV/cm and 10 microseconds duration applied at an interval of one sec., was 2.44 x 10(2) transformants/micrograms DNA and 3.16 x 10(-6) respectively. The saturated concentration of DNA absorbed by the protoplast was 5 micrograms DNA/10(9) cells/ml. By means of this method, pJB417, a recombinant mosquito larvicide clone, was introduced into B. subtilis 168M and B. sphaericus Ts-1. The transformants of B. subtilis 168M with biocide activity were obtained, but the toxicity of B. sphaericus Ts-1 was not increased. |
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