电喷雾萃取电离技术在蛋白质分析中的应用及展望

电喷雾萃取电离技术(extractive electrospray ionization，EESI)是一种能灵敏地电离固体、液体、气体、黏性样品等复杂基体中痕量大分子和小分子的新兴软电离技术．在简要介绍EESI原理的基础上，着重综述其在蛋白质分析中的应用．与商品化质谱仪器配置的电喷雾电离源(electrospray ionization，ESI)不同，EESI能够在常压条件下最大程度地保留蛋白质在样品中的原始构象，并获得大量具有生物活性的蛋白质离子．由此可见，EESI及类似的技术在蛋白质芯片制备、高分辨率氢/氘交换质谱(蛋白质结构分析)、蛋白质计量等方面具有良好的应用前景．本文也对其发展趋势进行了展望．

The Application and Perspective of Extractive Electrospray Ionization on The Protein Analysis

The extractive electrospray ionization (EESI) technique is an emerging soft ionization technique and it can be used for the sensitive detection of large molecules and small molecules in solid, liquid, gas and viscous samples without sample pretreatment. Based on the brief introduction of the mechanism of EESI, its applications on the protein analysis were reviewed in detail. Compared to the commercial electrospray ionization (ESI) source, EESI maintains the native structure of protein and thus the biological activity of the protein to the most extent, which shows it can be potentially applied in the fields of preparation of protein microarray, high resolution hydrogen/deuterium exchange mass spectrometry (structure analysis of protein), and metrology for protein.