新月弯孢霉菌丝球对染料脱色作用的研究

研究了新月弯孢霉Curvularia lunata JQH-100液体培养时产生的菌丝球对多种染料的脱色能力。结果表明,多种染料在24h内的脱色率均达到80%以上,且菌丝球稳定性良好,可重复使用6次;以菌丝球对孔雀绿脱色效果为优化指标,正交实验优化获得制备菌丝球的最佳条件为:葡萄糖20g/L、硫酸铵5g/L、马铃薯200g/L、KH2PO43g/L、MgSO45mg/L、CuSO40.5mg/L、VB15mg/L及pH5、摇床转速120r/min。在上述优化后的基础培养基(不含MgSO4、CuSO4)中分别添加微量元素Cu2+、Mn2+、Mg2+或Ca2+制备的菌丝球,对孔雀绿脱色能力增强;添加Fe2+制备的菌丝球,对孔雀绿脱色能力下降;分别添加Zn2+、Al3+或Na+制备的菌丝球对孔雀绿脱色能力与对照相近。应用优化后培养条件制备的菌丝球处理含多种染料的混合废水,也获得了较好的脱色效果。

Dye decolorization by self-immobilized Curvularia lunata mycelial pellets

The decolorization ability of Curvularia lunata mycelial pellets, formed during the growth process was tested by using several synthetic dyes. The result showed that more than 80% of the tested dyes could be removed within 24h. The mycelial pellets demonstrated a good stability and could be used for 6 times. The decolorization percentage of malachite green was used as an index to optimize the production conditions for C. lunata mycelial pellets. The result of orthogonal experiment indicated that the optimal conditions were: glucose 20g/L, ammonium sulfate 5g/L, pH 5, potato 200g/L, KH2PO4 3g/L, MgSO4 5mg/L, CuSO4 0.5mg/L, VB1 5mg/L and rotation rate 120r/min. The optimized basal culture medium (without MgSO4, CuSO4) supplemented with microelements Cu2+, Mn2+, Mg2+ or Ca2+ had obvious promotion effect on decolorization of malachite green. In contrast, the medium added with Fe2+ caused a negative effect on decolorization. The mycelial pellets from the optimized basal culture medium supplemented with Zn2+, Al3+ or Na+ had a similar decolorization ability as compared with the contrast. Furthermore, the mycelial pellets prepared from optimized medium could efficiently decolorize wastewater containing dye mixtures.