| 斜生褐孔菌多糖组分的纯化及其生物活性研究 |
| |
| 引用本文： | 申进文 余海尤 霍云凤 王斌. 斜生褐孔菌多糖组分的纯化及其生物活性研究[J]. 菌物学报, 2009, 28(4): 564-570 |
| |
| 作者姓名： | 申进文 余海尤 霍云凤 王斌 |
| |
| 作者单位： | 河南农业大学生命科学学院,郑州,450002;河南科技学院生物工程系,新乡,453003 |
| |
| 基金项目： | 河南省重点科技攻关项目 |
| |
| 摘 要： | 对斜生褐孔菌子实体和菌丝体的多糖含量进行了测定,二者多糖含量分别为2.63%和2.12%。运用DEAE Sephacel和Sephadex G-200柱层析技术从子实体中纯化出B1、B2、B31和B32四种多糖组分,分别占子实体多糖总量的50.20%、32.25%、4.48%和6.61%;从菌丝体中纯化出M1、M2和M3三种多糖组分,分别占菌丝体多糖总量的48.23%、31.85%和10.91%。生物活性试验结果显示:七种纯化多糖组分的抗氧化性由强到弱依次为B1>M1>M2>B2>M3>B32>B31;B2和M2具有在体外抑制人体白血病细胞株K-562和肝癌SMMC772l细胞株生长的作用;B31、B32和M3具有降低四氧嘧啶糖尿病小鼠血糖的作用,而且对健康小鼠的血糖值并无影响。
|
| 关 键 词： | 药用菌 菌丝体 子实体 抗氧化 抑制肿瘤 降血糖 |
Purification of polysaccharide of Phaeoporus obliquus and the bioactivity of purified fractions |
| |
| SHEN Jin-Wen YU Hai-You HUO Yun-Feng WANG Bin. Purification of polysaccharide of Phaeoporus obliquus and the bioactivity of purified fractions[J]. Mycosystema, 2009, 28(4): 564-570 |
| |
| Authors: | SHEN Jin-Wen YU Hai-You HUO Yun-Feng WANG Bin |
| |
| Affiliation: | College of Life Science, Henan Agriculture University, Zhengzhou 450002, China |
| |
| Abstract: | The content of polysaccharide of fruiting body and mycelia of Phaeoporus obliquus were determined and their values were 2.63% and 2.12% respectively.The methods of DEAE Sephacel column chromatography and Sephades G-200 column chromatography were employed to purify the raw polysaccharide of fruiting body and mycelia.The results showed that there were four kinds of fractions in raw polysaccharide of fruiting body,and they were marked as B1,B2,B31 and B32 and their contents were 50.20%,32.25%,4.48% and 6.61% r... |
| |
| Keywords: | medicinal fungi fruiting body mycelia anti-oxidant action anti-tumor reducing blood sugar |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
|
点击此处可从《菌物学报》下载全文 |
|
