Thermal denaturation of B. subtilis DNA in H2O and D2O observed by electron microscopy |
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| Authors: | Vincenzo Izzo Sandro L Fornili and Lorenzo Cordone |
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| Institution: | Istituto di Fisica dell Universita and Gruppi Nazionali Struttura della Materia of the Consiglio Nazionale delle Ricerche, Via Archirafi 36, 90123 Palermo, Italy1 |
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| Abstract: | In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the “opening” of DNA as a function of temperature. The results support the hypothesis that D2O plays a stabilizing role on strands separation during thermal denaturation of DNA. |
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