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Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection |
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| Authors: | Barry A Pepers Menno H Schut Rolf HAM Vossen Gert-Jan B van Ommen Johan T den Dunnen Willeke MC van Roon-Mom |
| Affiliation: | (1) Center for Human and Clinical Genetics, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands;(2) Department of Neurology, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands;(3) Leiden Genome Technology Center, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands |
| Abstract: | BackgroundMethodologies like phage display selection, in vitro mutagenesis and the determination of allelic expression differences include steps where large numbers of clones need to be compared and characterised. In the current study we show that high-resolution melt curve analysis (HRMA) is a simple, cost-saving tool to quickly study clonal variation without prior nucleotide sequence knowledge. |
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