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Micropropagation of Cardiospermum Halicacabum
Authors:S. Babber  K. Mittal  R. Ahlawat  T.M. Varghese
Affiliation:(1) Department of Botany, CCS Haryana Agricultural University, Hisar-, 125004 Haryana, India
Abstract:The in vitro studies with Cardiospermum halicacabum indicated that the different explants, i.e cotyledon, hypocotyl, cotyledonary node, leaf, internode and node had the potential to produce calli on Murashige and Skoog (MS) medium supplemented with benzylaminopurine (BAP) and napthalene acetic acid (NAA). Calli of different explant origin showed variable growth responses on different BAP concentrations. The shoots were favourably formed from the calli of leaf and cotyledon explants. The maximum number of shoots were produced from calli subcultured on MS + BAP (17.8 µM). The roots were initiated on growth regulator free MS medium.
Keywords:benzylaminopurine  in vitro culture  medicinal plants  plantlets multiplication
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