TCR‐β repertoire analysis of antigen‐specific single T cells using a high‐density microcavity array |
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Authors: | Atsushi Arakaki Kaori Ooya Yasuto Akiyama Masahito Hosokawa Masaru Komiyama Akira Iizuka Ken Yamaguchi Tadashi Matsunaga |
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Affiliation: | 1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2‐24‐16, Naka‐cho Koganei, Tokyo 184‐8588, Japan;2. telephone: 81‐42‐388‐7020;3. fax: 81‐42‐385‐7713;4. Immunotherapy Division, Shizuoka Cancer Center Research Institute, 1007, Shimo‐nagakubo, Nagaizumi‐cho, Sunto‐gun, Shizuoka 411‐8777, Japan |
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Abstract: | The antigen specificity of cytotoxic T cells, provided by T‐cell receptors (TCRs), plays a central role in human autoimmune diseases, infection, and cancer. As the TCR repertoire is unique in individual cytotoxic T cells, a strategy to analyze its gene rearrangement at the single‐cell level is required. In this study, we applied a high‐density microcavity array enabling target cell screening of several thousands of single cells for identification of functional TCR‐β gene repertoires specific to melanoma (gp100) and cytomegalovirus (CMV) antigens. T cells expressing TCRs with the ability to recognize fluorescent‐labeled antigen peptide tetramers were isolated by using a micromanipulator under microscopy. Regularly arranged cells on the microcavity array eased detection and isolation of target single cells from a polyclonal T‐cell population. The isolated single cells were then directly utilized for RT‐PCR. By sequencing the amplified PCR products, antigen‐specific TCR‐β repertoires for gp100 and human cytomegalovirus antigens were successfully identified at the single‐cell level. This simple, accurate, and cost‐effective technique for single‐cell analysis has further potential as a valuable and widely applicable tool for studies on gene screening and expression analyses of various kinds of cells. Biotechnol. Bioeng. 2010;106: 311–318. © 2010 Wiley Periodicals, Inc. |
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Keywords: | microarray analytical devices single‐cell T‐cell receptor beta genes T‐cell antigen receptor specificity gene expression analysis |
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