An Alternative Standard for Trolox‐Equivalent Antioxidant‐Capacity Estimation Based on Thiol Antioxidants. Comparative 2,2′‐Azinobis[3‐ethylbenzothiazoline‐6‐sulfonic Acid] Decolorization and Rotational Viscometry Study Regarding Hyaluronan Degradation |
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Authors: | Eva Hrabárová Katarína Valachová Peter Rapta Ladislav ?oltés |
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Institution: | 1. Department of Glycochemistry, Institute of Chemistry, Slovak Academy of Sciences, SK‐84538 Bratislava (phone: +421‐2‐59410318);2. Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK‐84104 Bratislava;3. Institute of Physical Chemistry and Chemical Physics, Faculty of Chemical and Food Technology, SK‐81237 Bratislava |
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Abstract: | Comparison of the effectiveness of antioxidant activity of three thiol compounds, D ‐penicillamine, reduced L ‐glutathione, and 1,4‐dithioerythritol, expressed as a radical‐scavenging capacity based on the two independent methods, namely a decolorization 2,2′‐azinobis3‐ethylbenzothiazoline‐6‐sulfonic acid] assay and a rotational viscometry, is reported. Particular concern was focused on the testing of potential free‐radical scavenging effects of thiols against hyaluronan degradation, induced by hydroxyl radicals. A promising, solvent‐independent, antioxidative function of 1,4‐dithioerythritol, comparable to that of a standard compound, Trolox®, was confirmed by the 2,2′‐azinobis3‐ethylbenzothiazoline‐6‐sulfonic acid] assay. The new potential antioxidant 1,4‐dithioerythritol exhibited very good solubility in a variety of solvents (e.g., H2O, EtOH, and DMSO) and could be widely accepted and used as an effective antioxidant standard instead of a routinely used Trolox® on 2,2′‐azinobis3‐ethylbenzothiazoline‐6‐sulfonic acid] assay. |
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Keywords: | Thiols Antioxidant activity 2 2′ ‐Azinobis[3‐ethylbenzothiazoline‐6‐sulfonic acid] Hyaluronan degradation |
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