EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes |
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Authors: | Yong Yang Chaoxiong Tao Dongming Zhao Feng Li Wenchun Zhao Hua Wu |
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Institution: | 1. Department of Orthopedics, Tongji Hospital, Medical College, Huazhong University of Science and Technology, Wuhan, China;2. Navy University of Engineering, Wuhan, China |
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Abstract: | The use of electromagnetic fields (EMFs) to treat nonunion fractures developed from observations in the mid‐1900s. Whether EMF directly regulates the bone marrow mesenchymal stem cells (MSCs), differentiating into osteoblasts or adipocytes, remains unknown. In the present study, we investigated the roles of sinusoidal EMF of 15 Hz, 1 mT in differentiation along these separate lineages using rat bone marrow MSCs. Our results showed that EMF promoted osteogenic differentiation of the stem cells and concurrently inhibited adipocyte formation. EMF increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulated osteoblast‐specific mRNA expression of RUNX2, ALP, BMP2, DLX5, and BSP. In contrast, EMF decreased adipogenesis and inhibited adipocyte‐specific mRNA expression of adipsin, AP‐2, and PPARγ2, and also inhibited protein expression of PPARγ2. These observations suggest that commitment of MSCs into osteogenic or adipogenic lineages is influenced by EMF. Bioelectromagnetics 31:277–285, 2010. © 2009 Wiley‐Liss, Inc. |
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Keywords: | electromagnetic fields (EMF) pulsed electromagnetic fields (PEMF) bone marrow mesenchymal stem cells (MSCs) osteogenesis adipogenesis |
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