Phylogenetic analysis of the fecal flora of the wild pygmy loris |
| |
| Authors: | Xu Bo Huang Zun‐xi Wang Xiao‐yan Gao Run‐chi Tang Xiang‐hua Mu Yue‐lin Yang Yun‐Juan Shi Hui Zhu Li‐da |
| |
| Institution: | 1. School of Life Science, Yunnan Normal University, Kunming, China;2. Key Laboratory of Enzyme Engineering, Yunnan Normal University, Kunming, China;3. Key Laboratory of Yunnan for Biomass Energy and Biotechnology of the Environment, Yunnan, Kunming, China;4. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan, Kunming, China |
| |
| Abstract: | The bacterial diversity in fecal samples from the wild pygmy loris was examined with a 16S rDNA clone library and restriction fragment length polymorphism analysis. The clones were classified as Firmicutes (43.1%), Proteobacteria (34.5%), Actinobacteria (5.2%), and Bacteroidetes (17.2%). The 58 different kinds of 16S rDNA sequences were classified into 16 genera and 20 uncultured bacteria. According to phylogenetic analysis, the major genera within the Proteobacteria was Pseudomonas, comprising 13.79% of the analyzed clone sequences. Many of the isolated rDNA sequences did not correspond to known microorganisms, but had high homology to uncultured clones found in human feces. Am. J. Primatol. 72:699–706, 2010. © 2010 Wiley‐Liss, Inc. |
| |
| Keywords: | pygmy loris bacterial diversity 16S rDNA RFLP phylogenetic analysis |
|
|
