Purification of human antibodies from transgenic corn using aqueous two‐phase systems |
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Authors: | J‐W Lee D Forciniti |
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Institution: | Dept. of Chemical and Biological Engineering, Missouri University of Science and Technology, Rolla, MO 65409 |
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Abstract: | A recombinant human antibody expressed in corn was purified using aqueous two‐phase extraction. The antibody was an immunoglobulin G fully unglycosylated. Using systems of different compositions and/or pHs in each of one or two partitioning stages followed by one more stage in which the antibody was precipitated at the liquid/liquid interface facilitated the removal of different impurities in each stage. The best system yields a product 72% pure (22‐fold purification) with a yield of 49%. The optimum extraction was done in two partitioning stages followed by an interfacial precipitation stage using poly(ethylene)glycol/potassium phosphate systems. NaCl was added to the first stage to eliminate large molecular weight impurities. The pH in the first stage was kept at 6 but a pH of 8 was used in the second stage and in the precipitation stage. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 |
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Keywords: | antibody purification aqueous two‐phases PEG |
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