Resonance scattering spectral detection of ultratrace Hg(II) using herring sperm DNA modified nanogold probe as catalyst |
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Authors: | Guiqing Wen Aihui Liang Zhiliang Jiang Xianjiu Liao Jianfu Li Hesheng Jiang |
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Institution: | 1. Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin 541004, China;2. Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guilin University of Technology, Guilin 541004, China;3. Animal Science and Technology College, Guangxi University, Nanning 531004, China |
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Abstract: | Herring sperm DNA (hsDNA) was used to modify 10 nm nanogold to obtain a resonance scattering (RS) probe (AuhsDNA) for detection of Hg2+. In the presence of salt, Hg2+ interacts with AuhsDNA to form stable Hg2+–hsDNA complexes, and releases nanogold particles to form larger nanogold clusters that can be removed by membrane filtration. The excess AuhsDNA in the filtrate solution exhibits a catalytic effect on the reaction between hydroxylamine (NH2OH) and Cu(II). The excess AuhsDNA decreased with the addition of Hg2+, which led the RS intensity at 602 nm to decrease. The decreased RS intensity (Δl602 nm) had a linear response to Hg2+ concentration in the range of 0.4–400 nM, with a detection limit of 0.2 nM Hg2+. This RS method was applied for the detection of Hg2+ in water samples, with sensitivity, selectivity and simplicity. Copyright © 2009 John Wiley & Sons, Ltd. |
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Keywords: | herring sperm DNA modified nanogold probe nanocatalysis Cu2O particles resonance scattering assay Hg2+ |
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