Glutathione cycle in stable chronic obstructive pulmonary disease |
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Authors: | Vanja Radišić Biljak Lada Rumora Ivana Čepelak Dolores Pancirov Sanja Popović‐Grle Jasna Sorić Tihana Žanić Grubišić |
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Affiliation: | 1. Department for Laboratory Medicine, University Clinic for Diabetes, Endocrinology and Metabolic Diseases Vuk Vrhovac, Zagreb, Croatia;2. Department of Medical Biochemistry and Haematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia;3. Department for Pulmonology, General Hospital “Dr. Ivo Pedi?i?”, Petrinja, Croatia;4. University Hospital for Lung Diseases “Jordanovac”, Medical School, Zagreb, Croatia |
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Abstract: | Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation and oxidant/antioxidant imbalance. Glutathione is the most abundant cellular low‐molecular weight thiol and the glutathione redox cycle is the fundamental component of the cellular antioxidant defence system. Concentration of total glutathione and catalytic activities of glutathione peroxidase and glutathione reductase were determined in peripheral blood of patients (n = 109) and healthy subjects (n = 51). Concentration of total glutathione in patients was not changed in comparison to healthy controls. However, we found statistically significant difference between patients with moderate and severe disease stages. Glutathione reductase activity was increased, while glutathione proxidase activity was decreased in the patients with COPD, when compared to healthy controls. We found no significant difference in glutathione peroxidase and glutathione reductase activities between stages. Patients who smoked had lower concentration of total glutathione compared with former smokers and never‐smoking patients. Lung function parameters were inversely associated with glutathione level. Evidence is presented for differential modulation of glutathione peroxidase and glutathione reductase activities in peripheral blood of patients with stable COPD. We suppose that in addition to glutathione biosynthesis, glutathione reductase‐dependent regulation of the glutathione redox state is vital for protection against oxidative stress. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | chronic obstructive pulmonary disease glutathione peroxidase glutathione reductase oxidative stress smoking total glutathione |
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