诱茶尺蠖天敌寄生蜂的茶树挥发物的分离与鉴定

采用Tenax GC动态吸附法对茶树-茶尺蠖Ectropis obliqua (Prout)-单白绵绒茧蜂Apanteles sp.三重营养关系中茶树的新梢挥发性化合物进行了捕集，经GC、GC-MS鉴定结果表明，不同来源的挥发物的组成和数量是不同的，完整茶树新梢(IS)的挥发物有11种组份；机械损伤新梢(MDS)的挥发物有13种组份；茶树-茶尺蠖取食复合体(PHC)的挥发物有15种组份，而用经典的SDE法获得的新梢精油有13种组份，4种挥发物数量的大小顺序为SDE>MDS>PHC>IS。生物测定结果表明，无气味经历的雌单白绵绒茧蜂对IS和MDS的气味的选择作用无统计上的显著性，对机械损伤新梢+幼虫口腔分泌物(MDS+Reg), 幼虫取食损伤(LDS)和PHC的气味有较强的偏好选择性。

ISOLATION AND IDENTIFICATION OF TEA PLANT VOLATILES ATTRACTIVE TO TEA GEOMETRID PARASITOIDS

The volatiles emitted from the various treated tea shoots were dynamically entrapped by Tenax GC and identified by GC and GC MS. Results showed that the volatiles emitted from various treated tea shoots differed in composition and relative contents. Volatile emitted from intact tea shoots (IS) was composed of a blend of 11 components; while volatile emitted from mechanically damaged tea shoots (MDS) was a blend of 13 components and volatile from plant host feeding complex (PHC) was a blend of 15 components. Volatile extracted from tea shoots by SDE method was identified to be a blend of 13 components. The order of relative contents from above treatments is SDE>MDS>PHC>IS. Y tube olfactometer bioassay results showed that inexperienced female wasps had no preference to volatiles emitted from IS and MDS over clean air (control) statistically, while strong preference to volatiles emitted from MDS+Reg, LDS, and PHC over control was observed.