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A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates
Authors:Leavy Tanya M  Bertozzi Carolyn R
Institution:Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA.
Abstract:O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates.
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