A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates |
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| Authors: | Leavy Tanya M Bertozzi Carolyn R |
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| Institution: | Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA. |
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| Abstract: | O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates. |
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