Resistance to amphotericin B associated with defective sterol Δ8→7 isomerase in a Cryptococcus neoformans strain from an AIDS patient |
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| Authors: | Steven L Kelly David C Lamb Mark Taylor rew J Corran Brian C Baldwin William G Powderly |
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| Institution: | Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, Sheffield University, Sheffield S10 2UH, UK; Zeneca Agrochemicals, Jealott's Hill Station, Jealott's Hill, Research Station, Jelott's Hill, Bracknell RG12 6EY, UK; Box 8011, Washington University School of Medicine, 660 S. Euclid, St. Louis, MO 63110, USA |
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| Abstract: | Abstract Two Cryptococcus neoformans strains isolated from an AIDS patient were investigated, a pretreatment isolate (CN1) and a second isolate (CN3) following failure of fluconazole and amphotericin B treatment. No difference in fluconazole sensitivity, but relative resistance to amphotericin B was observed for CN3. The sterol composition of CN3 indicated a defect in sterol Δ 8→7 isomerase in this strain and depletion of ergosterol, the major sterol of the CN1. |
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| Keywords: | Resistance Amphotericin B Cryptococcus neoformans |
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