An alternative to radiation hybrid mapping for large-scale genome analysis in barley |
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Authors: | Email author" target="_blank">Ali?Masoudi-NejadEmail author Shuhei?Nasuda Marie-Therese?Bihoreau Robbie?Waugh Takashi?Ryu?Endo |
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Institution: | (1) Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-Cho, 606-8502 Sakyo-Ku, Kyoto, Japan;(2) Wellcome Trust Center for Human Genetics, University of Oxford, Oxford, UK;(3) Genome Dynamics, Scottish Crop Research Institute, Oxford, UK |
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Abstract: | The presence of a monosomic gametocidal chromosome (GC) in a barley chromosome addition line of common wheat generates structural
aberrations in the barley chromosome as well as in the wheat chromosomes of gametes lacking the GC. A collection of structurally
aberrant barley chromosomes is analogous to a panel of radiation hybrid (RH) mapping and is valuable for high-throughput physical
mapping. We developed 90 common wheat lines (GC lines) containing aberrant barley 7H chromosomes induced by a gametocidal
chromosome, 2C. DNAs isolated from these GC lines provided a panel of 7H chromosomal fragments in a wheat genetic background,
comparable with RH mapping panels in mammals. We used this 7H GC panel and the methodology for RH mapping to physically map
PCR-based barley markers, SSRs and AFLPs, onto chromosome 7H, relying on polymorphism between the 7H chromosome and the wheat
genome. We call this method GC mapping. This study describes a novel adaptation and combination of methods of inducing chromosomal
rearrangements to produce physical maps of markers. The advantages of the presented method are similar to RH mapping in that
non-polymorphic markers can be used and the mapping panels can be relatively easily obtained. In addition, mapping results
are cumulative when using the same mapping set with new markers. The GC lines will be available from the National Bioresources
Project-KOMUGI ().
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. |
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Keywords: | Gametocidal chromosomes Physical mapping Radiation hybrid mapping Barley addition lines |
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