Localization of Mannose, TV-Acetylglucosamine and Galactose in the Golgi Apparatus, Plasma Membranes and Cell Walls of Scenedesmus acuminatus

The localization of lectin binding sites in the Golgi apparatus,plasma membranes and cell walls of Scenedesmus acuminatus wasinvestigated by cytochemical electron microscopy. The lectinsused were concanavalin A (Con A), peanut agglutinin (PNA) andwheat germ agglutinin (WGA), all labeled with gold. Con A-goldparticles were deposited not on the Golgi apparatus, but onthe outer cell-wall layer. PNA-gold and WGA-gold particles weredeposited on distal Golgi cisternae and vesicles derived fromthe Golgi apparatus. Entire cell-wall layers were evenly labeledby PNA-gold. The plasma membrane and cytoplasmic regions closeto the plasma membrane were labeled with WGA-gold. The processingof oligosaccharide in the Golgi apparatus, plasma membranesand cell walls of Scenedesmus acuminatus is discussed in referenceto that reported for animal cells. (Received March 5, 1987; Accepted July 18, 1987)