Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells |
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Authors: | Zhao Guixiang Etherton Terry D Martin Keith R Vanden Heuvel John P Gillies Peter J West Sheila G Kris-Etherton Penny M |
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Affiliation: | Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA. |
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Abstract: | The effects of linoleic acid (LA), alpha-linolenic acid (ALA), and docosahexaenoic acid (DHA) were compared to that of palmitic acid (PA), on inflammatory responses in human monocytic THP-1 cells. When cells were pre-incubated with fatty acids for 2-h and then stimulated with lipopolysaccharide for 24-h in the presence of fatty acids, secretion of interleukin (IL)-6, IL-1beta, and tumor necrosis factor-alpha (TNFalpha) was significantly decreased after treatment with LA, ALA, and DHA versus PA (P < 0.01 for all); ALA and DHA elicited more favorable effects. These effects were comparable to those for 15-deoxy-delta12,14-prostaglandin J2 (15d-PGJ2) and were dose-dependent. In addition, LA, ALA, and DHA decreased IL-6, IL-1beta, and TNFalpha gene expression (P < 0.05 for all) and nuclear factor (NF)-kappaB DNA-binding activity, whereas peroxisome proliferator-activated receptor-gamma (PPARgamma) DNA-binding activity was increased. The results indicate that the anti-inflammatory effects of polyunsaturated fatty acids may be, in part, due to the inhibition of NF-kappaB activation via activation of PPARgamma. |
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Keywords: | n-6 and n-3 fatty acids Linoleic acid α-Linolenic acid Docosahexaenoic acid Inflammation Interleukin-6 Interleukin-1β Tumor necrosis factor-α NF-κB, PPARγ |
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