Preliminary studies on eicosanoid production by fish leucocytes, using GC-mass spectrometry |
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| Authors: | A F Rowley ‡ S E Barrow † T C Hunt |
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| Institution: | Biomedical and Physiological Research Group, School of Biological Sciences, University College of Swansea, Singleton Park, Swansea SA28PP, U.K.;Department of Clinical Pharmacology, Royal Postgraduate Medical School, Ducane Road, London W12 0HS, U.K. |
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| Abstract: | Culture supernatants from dogfish leucocytes, exposed to the Ca2+ ionophore A23187, were analyzed for eicosanoid production by gas chromatography-electron capture mass spectrometry. Consistently high levels of the prostaglandins (PG) D2, F2α and E2 were produced, while thromboxane B2 and leukotriene B4 were found in smaller amounts. No 6-oxo PGF1α, a degradation product of prostacyclin, or 13,14-dihydro-15-oxo-PGF2α, a metabolite of PGE2 and PGF2α, were detected. The results are compared with similar experiments using mammalian leucocytes. |
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