甘蔗胚性细胞团和愈伤组织某些性质的比较

在培养24天的生长周期中,胚性细胞团的分裂细胞数共出现三次高峰。第一次出现在继代后的第8天,第二次出现在第12天,第三次出现在第18天,其中以第二次高峰期的细胞分裂数量最多。愈伤组织中仅观察到两次高峰,第一次出现在第12天,第二次出现在第18天。细胞分裂数比胚性细胞团少。细胞分裂高峰出现得迟可能愈伤组织比胚性细胞团生长缓慢的一个重要因素。 不同细胞分裂高峰时期胚性细胞团的DNA和蛋白质均比相同时期的愈伤组织含量高,高峰期在第12和第18天,这与内部的DNA和蛋白质含量变化是一致的,这说明胚性细胞团具有较快分裂能力的原因之一。胚性细胞团的过氧化物酶同工酶带也多于愈伤组织,酶带染色较深,表示酶活性高。

COMPARISON OF CERTAIN CHARACTERISTICS OF EMBRYOIDS AND CALLUS OF SUGAR-CANE

In the culture growth period for twenty-four days, the cell numbers of division of embryoids appeared three peaks: the first on eighth days after subculture; the second on twelfth day;the third on eighteenth day. Among them the cell numbers of division of the second peak is the most. The cell numbers of division of callus only two peaks were observed: the first on twelfth day, the second on eighteenth day. The cell numbers of division of callus are less than embryoids, The cell division and division peak of callus appeared later than the embryoids, slow growth of the callus is likely an important cause.During different stages of cell division peaks the contents of DNA and protein of embryoids all along higher than same stage of callus, the peaks appeared at twelfth and eighteen day, this is in accordance with the change in contents of DNA and protein and a cause of faster division.peroxidase isozyme bands of embryoids are more than callus. The deeper colour of the peroxidase bands showed the high activity of the peroxidase.