粗毛栓菌漆酶的诱导及其对中性染料和有机磷农药的降解

研究了不同初始培养pH和培养时间下，不同诱导剂对粗毛栓菌产漆酶诱导特性的影响及漆酶对中性染料、有机磷农药的降解.结果表明： RB-亮蓝、ABTS和邻联甲苯胺对粗毛栓菌产漆酶均有不同程度的诱导作用,其中ABTS的诱导作用最好,其诱导的最适初始pH为4.0,最佳时间为13 d；所产漆酶的适宜反应温度为38 ℃,酶在40 ℃保温20 min仍可保留786%的酶活力,稳定性较好；在ABTS介导下,漆酶降解6种中性染料的最佳温度分别为30 ℃(中性黑、中性枣红、中性桃红、甲基橙)和60 ℃(中性深黄、甲酚红),最适pH分别为6.0(中性黑)、2.0(中性枣红、中性桃红)和4.0(甲基橙、中性深黄、甲酚红),最佳降解时间分别为6 h(甲基橙、中性深黄和甲酚红)、12 h(中性桃红)和24 h(中性枣红).而漆酶降解乐果、毒死蜱、敌百虫、哒嗪硫磷4种有机磷农药的最佳温度均为25 ℃,最佳降解时间为9 h,最佳pH分别为10.0(乐果、毒死蜱、哒嗪硫磷)和8.0(敌百虫).

Induce of laccase from Trametes gallica and its degradation on neutral dyes and organophosphorus pesticides

The characteristics of the induction of laccase in Trametes gallica under different initial cultural pH,incubation time by different inducers were discussed,as well as the effects of temperature,pH and time on laccase degradation of six dyes and four organophosphors.The results showed that RB-bright blue,ABTS and o-toluidine affected the production of laccase at different levels,and ABTS was the best inductive agent in our test conditions,whose optimal initial pH and incubation time were 4.0 and 13 days,res...