| 外源Spd对高温胁迫下黄瓜幼苗叶片膜脂过氧化及质子泵活性的影响 |
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| 引用本文： | 田婧,郭世荣,孙锦,王丽萍,阳燕娟,李斌. 外源Spd对高温胁迫下黄瓜幼苗叶片膜脂过氧化及质子泵活性的影响[J]. 应用生态学报, 2011, 22(12): 3252-3258 |
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| 作者姓名： | 田婧 郭世荣 孙锦 王丽萍 阳燕娟 李斌 |
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| 作者单位： | 南京农业大学园艺学院/农业部南方蔬菜遗传改良重点开放实验室,南京,210095 |
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| 基金项目： | 国家重点基础研究发展计划项目(2009CB119000); 农业部现代农业产业技术体系建设专项资金项目(CARS-25-C-03); 江苏省农业三项工程项目(SX(2010)087); 江苏省高校优势学科建设工程项目资助 |
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| 摘 要： | 以较为耐热的黄瓜品种‘津春4号’为试材,在人工气候箱中,采用石英砂培养加营养液浇灌的栽培方式,研究了叶面喷施外源亚精胺(Spd)对高温胁迫下黄瓜幼苗叶片膜脂过氧化程度、质子泵活性及其基因表达的影响.结果表明:高温胁迫下,外源Spd促进黄瓜幼苗株高、茎粗、干、鲜质量和叶面积显著增加,有效抑制叶片相对电导率、丙二醛(MDA)含量和脂氧合酶(LOX)活性的升高,有助于提高叶片细胞质膜和液泡膜H+ -ATPase活性,但在基因表达水平上无显著差异.外源Spd可显著降低黄瓜幼苗叶片膜脂过氧化程度,提高质子泵活性,从而稳定膜的结构和功能,缓解高温胁迫对黄瓜幼苗造成的伤害,提高幼苗对高温胁迫的耐性.
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| 关 键 词： | 黄瓜 高温胁迫 亚精胺 膜脂过氧化 质子泵活性 |
Effects of exogenous spermidine on lipid peroxidation and membrane proton pump activity of cucumber seedling leaves under high temperature stress |
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| TIAN Jing , GUO Shi-rong , SUN Jin , WANG Li-ping , YANG Yan-juan , LI Bin. Effects of exogenous spermidine on lipid peroxidation and membrane proton pump activity of cucumber seedling leaves under high temperature stress[J]. The journal of applied ecology, 2011, 22(12): 3252-3258 |
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| Authors: | TIAN Jing GUO Shi-rong SUN Jin WANG Li-ping YANG Yan-juan LI Bin |
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| Affiliation: | TIAN Jing,GUO Shi-rong,SUN Jin,WANG Li-ping,YANG Yan-juan,LI Bin(College of Horticulture,Nanjing Agricultural University/Ministry of Agriculture Key Laboratory of Southern Vegetable Crop Genetic Improvement,Nanjing 210095,China). |
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| Abstract: | Taking a relatively heat-resistant cucumber(Cucumis sativus) cultivar 'Jinchun No.4' as test material,a sand culture experiment was conducted in growth chamber to investigate the effects of foliar spraying spermidine(Spd) on the lipid peroxidation,membrane proton pump activity,and corresponding gene expression of cucumber seedling leaves under high temperature stress.Compared with the control,foliar spraying Spd increased the plant height,stem diameter,dry and fresh mass,and leaf area significantly,and inhi... |
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| Keywords: | cucumber high temperature stress spermidine lipid peroxidation proton pump activity. |
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