Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco,a high altitude saline wetland in northern Chile |
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Authors: | Cristina Dorador Annika Busekow Irma Vila Johannes F. Imhoff Karl-Paul Witzel |
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Affiliation: | 1.Max Planck Institute for Evolutionary Biology,Pl?n,Germany;2.Facultad de Ciencias,Universidad de Chile,Santiago,Chile;3.Leibniz-Institute of Marine Sciences,Kiel,Germany;4.Centro de Biotecnología,Universidad Católica del Norte, Universidad Católica del Norte,Antofagasta,Chile |
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Abstract: | We analyzed enrichment cultures of ammonia-oxidizing bacteria (AOB) collected from different areas of Salar de Huasco, a high altitude, saline, pH-neutral water body in the Chilean Altiplano. Samples were inoculated into mineral media with 10 mM NH4 + at five different salt concentrations (10, 200, 400, 800 and 1,400 mM NaCl). Low diversity (up to three phylotypes per enrichment) of beta-AOB was detected using 16S rDNA and amoA clone libraries. Growth of beta-AOB was only recorded in a few enrichment cultures and varied according to site or media salinity. In total, five 16S rDNA and amoA phylotypes were found which were related to Nitrosomonas europaea/Nitrosococcus mobilis, N. marina and N. communis clusters. Phylotype 1-16S was 97% similar with N. halophila, previously isolated from Mongolian soda lakes, and phylotypes from amoA sequences were similar with yet uncultured beta-AOB from different biofilms. Sequences related to N. halophila were frequently found at all salinities. Neither gamma-AOB nor ammonia-oxidizing Archaea were recorded in these enrichment cultures. |
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Keywords: | Ammonia-oxidizing bacteria 16S rDNA amoA gene Andean Altiplano Salinity |
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