Stability of the hybrid plasmid pIM138 and its curing by some eliminating agents |
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| Authors: | H. Braná O. Benada O. Navrátil K. Čejka J. Hubáček |
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| Affiliation: | 1. Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4
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| Abstract: | Hybrid plasmid pIM138 was constructed by insertion of a chromosomal fragment with the threonme operon fromEscherichia coli into the pBR322 vector. Molar mass of pIM138 was 2.8 Mg/mol. Heteroduplexes between pBR322 vector and pIM138 hybrid DNA molecules were prepared. The hybrid plasmid shows a high stability against the curing effect of rifampicin and clorobiocm inE. coli SK1590thr host. |
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