Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba |
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Authors: | An Zhenfeng Jing Wen Liu Youliang Zhang Wenhua |
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Affiliation: | College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, PR China. |
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Abstract: | H2O2 is an essential signal in absicic acid (ABA)-induced stomatalclosure. It can be synthesized by several enzymes in plants.In this study, the roles of copper amine oxidase (CuAO) in H2O2production and stomatal closure were investigated. ExogenousABA stimulated apoplast CuAO activity, increased H2O2 productionand [Ca2+]cyt levels in Vicia faba guard cells, and inducedstomatal closure. These processes were impaired by CuAO inhibitor(s).In the metabolized products of CuAO, only H2O2 could inducestomatal closure. By the analysis of enzyme kinetics and polyaminecontents in leaves, putrescine was regarded as a substrate ofCuAO. Putrescine showed similar effects with ABA on the regulationof H2O2 production, [Ca2+]cyt levels, as well as stomatal closure.The results suggest that CuAO in V. faba guard cells is an essentialenzymatic source for H2O2 production in ABA-induced stomatalclosure via the degradation of putrescine. Calcium messengeris an important intermediate in this process. Key words: Abscisic acid, calcium, copper amine oxidase, hydrogen peroxide, putrescine, stomatal closure, Vicia fabaReceived 13 October 2007; Revised 16 December 2007 Accepted 20 December 2007 |
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Keywords: | Abscisic acid calcium copper amine oxidase hydrogen peroxide putrescine stomatal closure Vicia faba |
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