Constraints to virus infection in Nicotiana benthamiana plants transformed with a potyvirus amplicon |
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Authors: | María Calvo Gabriela Dujovny Cristina Lucini Jesús Ortuño Josefa M Alamillo Carmen Simón-Mateo Juan José López-Moya Juan Antonio García |
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Affiliation: | 1.Centro Nacional de Biotecnología-CSIC,Campus de la Universidad Autónoma de Madrid,Spain;2.Facultad de Ciencias y Artes,Universidad Católica de ávila,Spain;3.Departamento de Fisiología Vegetal, Facultad de Ciencias,Universidad de Córdoba,Córdoba,Spain;4.Centre for Research in Agricultural Genomics CRAG, CSIC-IRTA-UAB,Spain |
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Abstract: | Background Plant genomes have been transformed with full-length cDNA copies of viral genomes, giving rise to what has been called 'amplicon' systems, trying to combine the genetic stability of transgenic plants with the elevated replication rate of plant viruses. However, amplicons' performance has been very variable regardless of the virus on which they are based. This has boosted further interest in understanding the underlying mechanisms that cause this behavior differences, and in developing strategies to control amplicon expression. |
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