Antimicrobial activity and biofilm formation inhibition of green tea polyphenols on human teeth |
| |
Authors: | Yun-Seok Cho Jay Jooyoung Oh and Kye-Heon Oh |
| |
Institution: | (1) Department of Biomedical Sciences, Section of Microbiology, Polytechnic University of Marche, Via Tronto 10/A, 60020 Ancona, Italy;(2) Department of Pharmaceutical Chemistry, School of Pharmacy, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy;(3) DIBIO, Genova University, Corso Europa 26, 16132 Genova, Italy |
| |
Abstract: | The antimicrobial effects and biofilm formation inhibition of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis L) were evaluated against 12 oral microorganisms. Effective antimicrobial activity against all microorganisms tested, including
Lactobacillus spp. (Lactobacillus acidophilus and Lactobacillus plantarum), Streptococcus spp. (Streptococcus mutans, Streptococcus sanguis, Streptococcus sobrinus, Streptococcus mitis, and Streptococcus salivarius), Staphylococcus aureus, Neisseria meningitidis, Escherichia coli, Enterobacter cloacae, Enterococcus faecalis, and Candida albicans, was shown at 2,000 μg/mL TPP within 5 min of incubation. Scanning electron microscopy (SEM) analysis revealed various morphological
changes, such as the presence of perforations, the formation of cell aggregates, and the leakage of cytoplasmic materials
from cells treated with TPP, depending on the bacteria. The potential role of TPP in biofilm formation inhibition on human
teeth was evaluated in BHI broth with 2 mixed strains of S. mutans and S. sanguis. SEM analysis showed biofilm formation on the surface of a tooth shaken only in saline solution, whereas almost no biofilm
was observed on a tooth incubated in TPP solution. This result suggests that TPP is effective against adherent cells of S. mutans and S. sanguis. Thus, TPP would be useful for development as an antimicrobial agent against oral microorganisms, and has great potential
for use in mouthwash solutions for the prevention and treatment of dental caries. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|