In vitro regeneration from cotyledon explants in figleaf gourd (<Emphasis Type="Italic">Cucurbita ficifolia</Emphasis> Bouché), a rootstock for Cucurbitaceae |
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Authors: | Kyung-Min Kim Chang Kil Kim Jeung-Sul Han |
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Institution: | (1) College of Ecology and Environmental Science, Kyungpook National University, Sangju, 742-711, Republic of Korea; |
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Abstract: | An efficient plant regeneration system has been developed for figleaf gourd (Cucurbita ficifolia Bouché), which is exclusively used as a rootstock for cucumber. The protocol is based on results obtained from a series of
culture experiments involving different parts of the cotyledons and various media. The culture of cotyledon explants was critical
for the enhancement of shoot regeneration frequency. The lower parts of the cotyledon excised at the plumule base were found
to display a markedly enhanced production of adventitious shoots compared to other cotyledon regions. Culture in silver nitrate-supplemented
Murashige and Skoog (MS) medium was not beneficial for shoot regeneration and suppressed root regeneration. Efficient shoot
regeneration was obtained on MS medium containing 1.0 mg l−1 zeatin and 0.1 mg l−1 indole-3-acetic acid. Regenerated shoots successfully elongated and rooted in medium containing 0.1 mg l−1 1-naphthaleneacetic acid after 10–15 days of subculturing. The plantlets were satisfactorily acclimatized in a greenhouse
and grew into normal plants without any morphological alterations. |
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