Enhancement of autofluorescence of the brown-rot fungus <Emphasis Type="Italic">Piptoporus betulinus</Emphasis> by metal ions |
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| Authors: | Z Žižka T Vêtrovský J Gabriel |
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| Institution: | (1) Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;(2) Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;(3) Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland |
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| Abstract: | The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with
glucose, where the hyphae exhibited green AF at violet (450 nm) excitation and red AF at green (570 nm) excitation. Addition
of metals to cultivation media led to enhanced white-blue AF in the presence of Co (at 450 nm) and yellow to yellow-brown
AF at 510 nm. When cultivated with Mn and Zn, enhanced AF of intracellular content was observed. Only a weak signal was found
in the presence of Cu and Fe. |
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