Cholecystokinin octapeptide (CCK 26–33), nonsulfated octapeptide and tetrapeptide (CCK 30–33) in rat brain: Analysis by high pressure liquid chromatography (HPLC) and radioimmunoassay (RIA)

Cholecystokinin carboxyterminal octa- and tetrapeptide concentrations have been measured in rat brain by a combination of high pressure liquid chromatography and radioimmunoassay. The sulfated octapeptide is the predominant form of Cholecystokinin in rat brain (approx. 80%). The concentration of the tetrapeptide represents 5–10% of that of the sulfated octapeptide in molar terms, depending upon the brain region. In addition to the tetrapeptide, a peptide with the properties of Cholecystokinin octapeptide in its nonsulfated form could be detected in concentrations similar to those of the tetrapeptide.