The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea |
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| Authors: | S. Kiran Ghanti K. G. Sujata M. Srinath Rao |
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| Affiliation: | (1) National Research Centre on Plant Biotechnology, New Delhi, India;(2) Present address: Navasari Agricultural University, Navasari, India;(3) Present address: ICAR Research Complex, Umiam, Meghalaya, India; |
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| Abstract: | A highly efficient protocol for plant regeneration from cotyledonary node of two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri used phenylacetic acid (PAA). The Murashige and Skoog (MS) medium supplemented with 2.0 mg dm−3 6-benzylaminopurine (BAP) and 1.0 mg dm−3 PAA was used for induction of bud formation. Buds were elongated on MS medium supplemented either with only 0.75 mg dm−3 gibberellic acid (GA3) or 0.2 mg dm−3 GA3 + 0.6 mg dm−3 PAA. The elongated shoots were then transferred onto rooting medium containing 1 mg dm−3 PAA. The frequency of multiple shoot induction and rooting was higher in Annigeri as compared to ICCV-10. The complete plantlets with well-developed roots were transferred to pots containing sterilized soil and sand in the ratio 3:1 where they survived (74 %) and set normal seeds. |
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