A new procedure for data reduction in electrophoretic fingerprints of whole-cell proteins |
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Authors: | Paolo Piraino Annamaria Ricciardi Maria Teresa Lanorte Iana Malkhazova Eugenio Parente |
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Affiliation: | (1) Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Potenza, Italy;(2) Eliava Phage Institute, Tblisi, Georgia |
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Abstract: | A procedure for the treatment of electrophoretic patterns is presented and discussed using SDS-PAGE patterns of whole-cell proteins of lactic acid bacteria as an example. Complex patterns consisting of two vectors of data [molecular weights (MW), and band intensities] with varying length were transformed in a single vector of fixed length consisting of band intensities accumulated in classes of MW by a logistic weighting function. The procedure performed better than a commercial software (Diversity Database) in clustering the patterns. |
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Keywords: | classification electrophoretic patterns fingerprinting numerical analysis SDS-PAGE |
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