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A new procedure for data reduction in electrophoretic fingerprints of whole-cell proteins
Authors:Paolo Piraino  Annamaria Ricciardi  Maria Teresa Lanorte  Iana Malkhazova  Eugenio Parente
Affiliation:(1) Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Potenza, Italy;(2) Eliava Phage Institute, Tblisi, Georgia
Abstract:A procedure for the treatment of electrophoretic patterns is presented and discussed using SDS-PAGE patterns of whole-cell proteins of lactic acid bacteria as an example. Complex patterns consisting of two vectors of data [molecular weights (MW), and band intensities] with varying length were transformed in a single vector of fixed length consisting of band intensities accumulated in classes of MW by a logistic weighting function. The procedure performed better than a commercial software (Diversity Database) in clustering the patterns.
Keywords:classification  electrophoretic patterns  fingerprinting  numerical analysis  SDS-PAGE
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