HCV复制子与细胞培养体系研究进展

丙型肝炎是由丙型肝炎病毒(hepatitis C virus,HCV)感染所导致的传染性肝病,呈现世界性流行态势,严重危害人类健康。由于病毒自身高度突变,以及广泛高效的细胞培养体系和合适的小动物模型缺乏,目前尚无可有效预防的疫苗。自1989年丙型肝炎病毒基因组首次被确定以来,Con1(1b)亚基因组复制子和JFH1(2a)毒株细胞培养体系相继建立。以此为工具,HCV生活周期多个关键环节得以阐明。近年来,研究者在Con1亚基因组复制子、JFH1和J6/JFH1细胞培养体系的基础上,构建出多个基因型和亚型的复制子和细胞培养体系。不同的体系在HCV复制与致病机制研究、抗病毒药物筛选方面,具有不同的用途及优缺点。针对HCV复制子与细胞培养体系的研究进展进行综述,可为HCV的相关研究提供参考。

The development of HCV replicon and cell culture system

Hepatitis C is an infectious hepatopathy caused by hepatitis C virus （HCV）. It is globally prevalent and seriously harmful to the health of human beings. So far, there are no preventive vaccines due to the lack of efficient cell culture systems derived from various genotypes and suit small animal models, and also the frequent mutation of HCV genome. Since the genome of HCV firstly was determined in 1989 using molecular cloning technique, the replicons of Conl subgenome and JFH1 （2a） cell culture system were constructed subsequently. Further studies made great progress in our understanding on the multiple phases of the life cycle of this virus. Recently, based on the constructing strategies of the Conl replicon, JFH1 and J6/JFH1 cell culture systems, HCV replicons and cell culture systems of other genotypes have been generated. Various systems have been selectively used in the research of HCV replication, pathogenic mechanism and anti-virus drug screening. Here, we review the progress in the construction ofHCV replicons and cell culture systems to provide a helpful reference for the research of HCV.