Cytotoxity of the <Emphasis Type="Italic">trans</Emphasis>10,<Emphasis Type="Italic">cis</Emphasis>12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids,tocopherol, and tocotrienol |
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Authors: | Email author" target="_blank">Masao?YamasakiEmail author Shinsuke?Nou Hrofumi?Tachibana Koji?Yamada |
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Institution: | (1) Department of Biochemistry and Applied BioScience, Faculty of Agriculture, Miyazaki University, 1-1 Gakuenkibanadai-nishi, 889-2192 Miyazaki, Japan;(2) Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581 Fukuoka, Japan;(3) R&D Division, Omu Milk Products Co., Ltd, 1-38-1 Shin Katsudachi Omuta, 836-0895 Fukuoka, Japan |
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Abstract: | Summary This study was performed to evaluate the isomer-specific cytotoxic effects of conjugated linoleic acid (CLA) on rat hepatoma
dRLh-84 cells in vitro. A 10trans,12cis (10t,12c)-CLA showed a strong cytotoxic effect on dRLh-84 cells in culture, whereas no such effect was observed with 9cis,11trans (9c,11t)-CLA or linoleic acid. The optimum concentration for induction of cytotoxity by 10t,12c-CLA was 5 to 10 μM, but the effect was alleviated at higher concentrations. Coincubation with oleic or palmitoleic acid and 10t,12c-CLA cancelled the cytotoxic effect, but other major saturated or polyunsaturated fatty acids and eraidic acid did not interfere
with 10t,12c-CLA-induced cytotoxity. The cytotoxic effect was also alleviated by α-tocopherol (α-toc) and α-tocotrienol but not by any
other antioxidant regent examined. Significant cytotoxity of 10t,12c-CLA was detected after only a 15-min incubation, and the most noticeable effect was seen after 3 h. After incubation with
10t,12c-CLA at 10 μM, an additional 90 μM, an additional 90 μM of 10t,12c-CLA or 100 μM of α-toc was also able to alleviate the cytotoxity. When cells were treated with 10 μM 10t,12c-CLA for more than 48 h, treatment with additional CLA or α-toc could not prevent cell death. |
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Keywords: | conjugated linoleic acid oleic acid palmitoleic acid α -tocopherol α -tocotrienol hepatoma |
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